Effect of Blood Storage on Biochemical Parameters Assessed at Periodic Intervals in CPDA1 Blood Bags

Abstract

Introduction: Blood when stored at 4°C, effectuates alterations in biochemical and mechanical properties of red blood cells because of storage conditions, which are called as storage lesions. Red blood cells lose their viability over a period of time even after storing blood with Citrate Phosphate Dextrose Adenine (CPDA-1) anticoagulant. Aim: To observe the changes in biochemical parameters namely Glucose, Urea, Creatinine, Total protein, Aspartate Transaminase (AST), Albumin, Sodium, Potassium and Chloride in stored blood. Materials and Methods: The present study was a prospective study conducted on blood samples donated by 50 healthy voluntary donors. Biochemical parameters namely Glucose, Urea, Creatininie, Total protein, AST, Albumin, Sodium, Potassium and Chloride were estimated in stored blood collected in CPDA1 blood bags on 0,3,7,14 and 21st day. All the data were analysed using SPSS 22.0 software. Student's t-test was used to find the effect of blood storage on its biochemical parameters. Correlation analysis was used to find the relationship between biochemical parameters and period of storage. Results: Significant changes were observed in serum AST, Total protein, Albumin, Urea, Chloride, and Potassium levels (p-value <0.05). Rest of the biochemical parameters did not show any significant change over the period of storage time. Conclusion: In the present study, authors observed that there were significant changes in some of the biochemical parameters namely serum AST, Total protein, Albumin, Urea, Chloride, and Potassium levels (p-value<0.05) assessed at periodic intervals. The changes might be due to spontaneous haemolysis or non viable red blood cells caused due to storage. Therefore, it is better to transfuse blood as early as possible in order to prevent a negative impact on the biochemical composition of red blood cells. Also, usage of CPDA2 or SAGM anticoagulant solutions may to some extent minimise this issue. The further contrivance of newer anticoagulant solution in blood bags can be focused upon to reduce the impact of storage.

Keywords

• red blood cell
• haemolysis
• transfusion