Materials Proceedings (May 2023)

Neuron and Glial Cells Exposed to Cerium Dioxide Nanoparticles: Results from MTT and γH2AX Assays

  • Natalia Fernández-Bertólez,
  • Assia Touzani,
  • Luisa Martínez,
  • Josefina Méndez,
  • Ana Teresa Reis,
  • Carla Costa,
  • Sonia Fraga,
  • João Paulo Teixeira,
  • Eduardo Pásaro,
  • Blanca Laffon,
  • Vanessa Valdiglesias

DOI
https://doi.org/10.3390/IOCN2023-14517
Journal volume & issue
Vol. 14, no. 1
p. 4

Abstract

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Cerium dioxide nanoparticles (CeO2NP) show antioxidant enzyme-like properties and reactive oxygen species (ROS) scavenging activity, making them a promising material for potential therapeutic applications in neurodegenerative diseases. The objective of this work was to assess the biological behavior of CeO2NP in human SH-SY5Y neuronal and A172 glial cells by means of the MTT assay and the γH2AX assay. Despite the significant dose- and time-dependent NP internalization by both cell lines, nanoceria generally presented scarce cytotoxicity or genotoxicity, essentially restricted to the highest NP doses and longest exposure times. In conclusion, a high biocompatibility of CeO2NP was observed under the conditions tested.

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