“Smart” Polylactic Acid Films with Ceftriaxone Loaded Microchamber Arrays for Personalized Antibiotic Therapy
Ekaterina A. Mordovina,
Valentina O. Plastun,
Arkady S. Abdurashitov,
Pavel I. Proshin,
Svetlana V. Raikova,
Daniil N. Bratashov,
Olga A. Inozemtseva,
Irina Yu. Goryacheva,
Gleb B. Sukhorukov,
Olga A. Sindeeva
Affiliations
Ekaterina A. Mordovina
Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia
Valentina O. Plastun
Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia
Arkady S. Abdurashitov
Center for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, 3 Nobel Str., 143005 Moscow, Russia
Pavel I. Proshin
Center for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, 3 Nobel Str., 143005 Moscow, Russia
Svetlana V. Raikova
Saratov Hygiene Medical Research Center of the FBSI «FSC Medical and Preventive Health Risk Management Technologies», 1A Zarechnaya Str., 410022 Saratov, Russia
Daniil N. Bratashov
Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia
Olga A. Inozemtseva
Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia
Irina Yu. Goryacheva
Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia
Gleb B. Sukhorukov
Center for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, 3 Nobel Str., 143005 Moscow, Russia
Olga A. Sindeeva
Center for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, 3 Nobel Str., 143005 Moscow, Russia
Bacterial infections are a severe medical problem, especially in traumatology, orthopedics, and surgery. The local use of antibiotics-elution materials has made it possible to increase the effectiveness of acute infections treatment. However, the infection prevention problem remains unresolved. Here, we demonstrate the fabrication of polylactic acid (PLA) “smart” films with microchamber arrays. These microchambers contain ceftriaxone as a payload in concentrations ranging from 12 ± 1 μg/cm2 to 38 ± 8 μg/cm2, depending on the patterned film thickness formed by the different PLA concentrations in chloroform. In addition, the release profile of the antibiotic can be prolonged up to 72 h in saline. At the same time, on the surface of agar plates, the antibiotic release time increases up to 96 h, which has been confirmed by the growth suppression of the Staphylococcus aureus bacteria. The efficient loading and optimal release rate are obtained for patterned films formed by the 1.5 wt % PLA in chloroform. The films produced from 1.5 and 2 wt % PLA solutions (thickness—0.42 ± 0.12 and 0.68 ± 0.16 µm, respectively) show an accelerated ceftriaxone release upon the trigger of the therapeutic ultrasound, which impacted as an expansion of the bacterial growth inhibition zone around the samples. Combining prolonged drug elution with the on-demand release ability of large cargo amount opens up new approaches for personalized and custom-tunable antibacterial therapy.