Cytotoxicity and degree of conversion of methacrylate and silorane

Abstract

Composites have been proven to have a cytotoxiceffect on a variety of tissues and cells. Aim: The aim of thisstudy was to analyse the degree of conversion of resinsand its correlation with the cell viability in primary gingivalfibroblasts. Methods: Resin-based silorane (Filtek P90)and conventional methacrylate resins (Filtek Z100, FiltekZ250 and Filtek Z350XT) were used to evaluate cell viabilityand the degree of conversion. The resins were light-cured bya LED for 20 and 40 seconds. The degree of conversion wasanalysed by Fourier transform infrared spectroscopy. Cellularmetabolism was evaluated after 24 hours by the MTT assay(n = 6) using the storage solution of composite resin foreither 24 hours or 12 days. Variance analysis (ANOVA) witha Bonferroni correction (p < 0.05) was performed to comparethe groups. Results: The composite Filtek P90 showed ahigher degree of conversion when polymerised for 40 or20 seconds, while the composites Filtek Z100, Filtek Z250 andFiltek Z350XT showed similar degree of conversion. Only theFiltek Z100 resin was cytotoxic. Conclusion: We found nostatistically significant correlation between cell viability andthe degree of conversion.

Keywords

• Composite resins
• Cytotoxins
• Fibroblasts