Genotypic Analysis of Aroma Gene Badh2 in Aromatic Rice Cultivars in South China

Xueyu LIANG; Min GUO; Xuhua OU; Ying CHEN; Danhua ZHOU; Cuihong HUANG; Hui WANG; Guili YANG

doi:10.16768/j.issn.1004-874X.2023.12.010

Guangdong nongye kexue (Dec 2023)

Genotypic Analysis of Aroma Gene Badh2 in Aromatic Rice Cultivars in South China

Xueyu LIANG,
Min GUO,
Xuhua OU,
Ying CHEN,
Danhua ZHOU,
Cuihong HUANG,
Hui WANG,
Guili YANG

Affiliations

Xueyu LIANG: Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525099, China
Min GUO: Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525099, China
Xuhua OU: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China
Ying CHEN: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China
Danhua ZHOU: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China
Cuihong HUANG: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China
Hui WANG: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China
Guili YANG: Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525099, China

DOI: https://doi.org/10.16768/j.issn.1004-874X.2023.12.010
Journal volume & issue: Vol. 50, no. 12
pp. 104 – 111

Abstract

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【Objective】Aroma is one of the important quality traits of rice, which directly affects consumers' evaluation on cooking and eating quality of rice. The selection of aromatic rice resources and the identification and utilization of aroma genes has practical significance for the genetic breeding and the development of aromatic rice. The aroma gene, Badh2, is a major gene that has been cloned to control the aroma of rice. Clarifying the distribution and utilization frequency of different alleles of Badh2 in main aromatic rice varieties planted in South China will better serve the breeding practice of aromatic rice in this area.【Method】Genotypic analysis was conducted on Badh2 in 30 rice cultivars collected from different areas in South China with the KASP functional molecular marker. The aroma of all the cultivars were tested with different evaluation methods including chewing method, KOH soaking method and HS-SPME/GC-MS. Meanwhile, the aroma ancestries of the above aromatic rice cultivars were traced, and their genetic backgrounds of aroma origins were analysed.【Result】KASP molecular markers could accurately genotype the aroma gene Badh2. The Badh2 allele variations of 30 aromatic rice varieties planted in South China were all Badh2-E7 type. The results of the aroma test showed that all Badh2-E7 type varieties possessed aroma. The aroma source parent tracing analysis of the aromatic rice varieties revealed that the aroma source parents of aromatic rice varieties were relatively simple and mainly included Xiangyaxiangzhan, Basmati 370, and etc. Xiangyaxiangzhan accounted for about 30% of the aroma source parents, and Basmati 370 accounted for about 13% of the aroma source parents.【Conclusion】Large scale and rapid identification of aromatic rice can be achieved through KASP genotyping technology. The Badh2 alleles of 30 aromatic rice varieties collected in South China are all the genotype of Badh2-E7, and the simplicity of the genetic background of the aroma gene may result from the single source of the aroma parent. In future aromatic rice breeding, new aroma genes can be introduced to enrich the genetic background of rice aroma.

Published in Guangdong nongye kexue

ISSN: 1004-874X (Print)
Publisher: Guangdong Academy of Agricultural Sciences
Country of publisher: China
LCC subjects: Agriculture
Website: http://gdnykx.cnjournals.org/gdnykx/ch/index.aspx

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