Generation of induced pluripotent stem cell line (XWHNi002-A) from a female with APP gene mutation

Wenxian Sun; Pin Wang; Jin Gong; Yufei Chen; Yuting Yang; Heya Luan; Shaoqi Li; Ruina Li; Cuibai Wei

Stem Cell Research (Sep 2023)

Generation of induced pluripotent stem cell line (XWHNi002-A) from a female with APP gene mutation

Wenxian Sun,
Pin Wang,
Jin Gong,
Yufei Chen,
Yuting Yang,
Heya Luan,
Shaoqi Li,
Ruina Li,
Cuibai Wei

Affiliations

Wenxian Sun: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Pin Wang: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Jin Gong: College of Integrated Traditional Chinese and Western Medicine, Changchun University of Chinese Medicine, Changchun, China
Yufei Chen: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Yuting Yang: School of Biological Science and Medical Engineering, Beihang University, Beijing, China
Heya Luan: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
Shaoqi Li: College of Integrated Traditional Chinese and Western Medicine, Changchun University of Chinese Medicine, Changchun, China
Ruina Li: School of Biological Science and Medical Engineering, Beihang University, Beijing, China
Cuibai Wei: Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China; Corresponding author.

Journal volume & issue: Vol. 71
p. 103149

Abstract

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The β-amyloid precursor protein (APP) is a crucial pathogenic gene linked to Alzheimer's disease (AD). A human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMCs) isolated from a female with APP gene mutation utilizing non-integrative Sendai virus. The iPSC line exhibits high expression of pluripotency markers, retains the APP mutation, displays a normal karyotype, and has the ability to differentiate into normal teratoma tissue. This iPSC line represents a valuable cell model for investigating the pathological mechanisms and therapeutic strategies of AD.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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