Nature Communications (Aug 2022)
Engineering an autonomous VH domain to modulate intracellular pathways and to interrogate the eIF4F complex
- Yuri Frosi,
- Yen-Chu Lin,
- Jiang Shimin,
- Siti Radhiah Ramlan,
- Kelly Hew,
- Alf Henrik Engman,
- Anil Pillai,
- Kit Yeung,
- Yue Xiang Cheng,
- Tobias Cornvik,
- Par Nordlund,
- Megan Goh,
- Dilraj Lama,
- Zachary P. Gates,
- Chandra S. Verma,
- Dawn Thean,
- David P. Lane,
- Ignacio Asial,
- Christopher J. Brown
Affiliations
- Yuri Frosi
- p53 Laboratory (A*STAR)
- Yen-Chu Lin
- p53 Laboratory (A*STAR)
- Jiang Shimin
- p53 Laboratory (A*STAR)
- Siti Radhiah Ramlan
- p53 Laboratory (A*STAR)
- Kelly Hew
- DotBio Pte. Ltd., 1 Research Link
- Alf Henrik Engman
- DotBio Pte. Ltd., 1 Research Link
- Anil Pillai
- DotBio Pte. Ltd., 1 Research Link
- Kit Yeung
- DotBio Pte. Ltd., 1 Research Link
- Yue Xiang Cheng
- DotBio Pte. Ltd., 1 Research Link
- Tobias Cornvik
- School of Biological Sciences, Nanyang Technological University
- Par Nordlund
- DotBio Pte. Ltd., 1 Research Link
- Megan Goh
- p53 Laboratory (A*STAR)
- Dilraj Lama
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Zachary P. Gates
- Disease Intervention Technology Laboratory (DITL), Institute of Molecular and Cell Biology, A*STAR
- Chandra S. Verma
- School of Biological Sciences, Nanyang Technological University
- Dawn Thean
- p53 Laboratory (A*STAR)
- David P. Lane
- p53 Laboratory (A*STAR)
- Ignacio Asial
- DotBio Pte. Ltd., 1 Research Link
- Christopher J. Brown
- p53 Laboratory (A*STAR)
- DOI
- https://doi.org/10.1038/s41467-022-32463-1
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 22
Abstract
Approaches have been devised to increase the discovery rate of intrabodies but often these yield results that aren’t functional in cells. Here the authors engineer and optimise an autonomous and disulphide-free human VH domain for intracellular expression, and they identify several VH domain binders against eIF4E.
