Hsa_circRNA_0084043 promoting tumorigenesis in glioma through miR-577 sponging
Jin Qian,
Chunyan Chen,
Changping Zhao,
Xing Xu,
Yingna Xu,
Yong Zhang,
Chunfa Qian
Affiliations
Jin Qian
Department of Neurosurgery, Xuancheng People's Hospital, The Affiliated Xuancheng Hospital of Wannan Medical College, Xuancheng, Anhui, China; Corresponding author. Department of Neurosurgery, Xuancheng People's Hospital, NO.51 Dabatang Road, 242099, Xuancheng, Anhui, China.
Chunyan Chen
Department of Gastroenterology and Hepatology, Jinling Hospital, Southern Medical University, Nanjing, China
Changping Zhao
Department of Neurosurgery, Xuancheng People's Hospital, The Affiliated Xuancheng Hospital of Wannan Medical College, Xuancheng, Anhui, China
Xing Xu
Department of Neurosurgery, Xuancheng People's Hospital, The Affiliated Xuancheng Hospital of Wannan Medical College, Xuancheng, Anhui, China
Yingna Xu
Department of Neurosurgery, Xuancheng People's Hospital, The Affiliated Xuancheng Hospital of Wannan Medical College, Xuancheng, Anhui, China
Yong Zhang
Department of Neurosurgery, Xuancheng People's Hospital, The Affiliated Xuancheng Hospital of Wannan Medical College, Xuancheng, Anhui, China
Chunfa Qian
Department of Neurosurgery, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu, China; Corresponding author. Department of Neurosurgery, Affiliated Nanjing Brain Hospital, Nanjing Medical University, NO.264 Guangzhou Road, 210029, Nanjing, Jiangsu, China.
Circular RNAs (circRNAs) are important non-coding RNAs (ncRNAs) involved in the development of multiple human diseases, especially cancers. circRNA_0084043 is significantly involved in the progression of melanoma. However, whether circRNA_0084043 is associated with glioma remains unknown. In this study, the upregulation of circRNA_0084043 in glioma and the association between circRNA_0084043 and glioma grade were identified. Our results showed that circRNA_0084043 is significantly involved in the proliferative, migratory, and invasive capacities of glioma cells. The results obtained from starBase, luciferase reporter assays, RNA immunoprecipitation assays, and RNA pull-down assays demonstrated that circRNA_0084043 acts as a direct sponge for miR-577. TargetScan algorithm was used to identify potential miR-577 targets, it was found that sorting nexin 5 (SNX5) is a candidate bound to miR-577. Finally, cell experiments testified that circRNA_0084043 enhanced growth, migration and invasion of glioma through the regulation of miR-577-mediated SNX5. Taken together, we concluded that circRNA_0084043 in the miR-577/SNX5 axis can be used as a candidate target for glioma therapy.
