Characterization of human umbilical cord blood-derived mast cells using high-throughput expression profiling and next-generation knowledge discovery platforms

Sherin Bakhashab; Ghalya H. Banafea; Farid Ahmed; Haneen Alsehli; Huda F. AlShaibi; Nadia Bagatian; Ohoud Subhi; Kalamegam Gauthaman; Mahmood Rasool; Hans-Juergen Schulten; Peter Natesan Pushparaj

Experimental and Molecular Pathology (Aug 2023)

Characterization of human umbilical cord blood-derived mast cells using high-throughput expression profiling and next-generation knowledge discovery platforms

Sherin Bakhashab,
Ghalya H. Banafea,
Farid Ahmed,
Haneen Alsehli,
Huda F. AlShaibi,
Nadia Bagatian,
Ohoud Subhi,
Kalamegam Gauthaman,
Mahmood Rasool,
Hans-Juergen Schulten,
Peter Natesan Pushparaj

Affiliations

Sherin Bakhashab: Biochemistry Department, King Abdulaziz University, Jeddah, Saudi Arabia; Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia; Corresponding authors at: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.
Ghalya H. Banafea: Biochemistry Department, King Abdulaziz University, Jeddah, Saudi Arabia
Farid Ahmed: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
Haneen Alsehli: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Centre for Stem Cells & Regenerative Medicine, King's College London, UK
Huda F. AlShaibi: Biochemistry Department, King Abdulaziz University, Jeddah, Saudi Arabia
Nadia Bagatian: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia
Ohoud Subhi: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia
Kalamegam Gauthaman: Center for Transdisciplinary Research, Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India
Mahmood Rasool: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
Hans-Juergen Schulten: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
Peter Natesan Pushparaj: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Center for Transdisciplinary Research, Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India; Corresponding authors at: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.

Journal volume & issue: Vol. 132
p. 104867

Abstract

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Mast cells (MCs) are tissue-resident innate immune cells that express the high-affinity receptor for immunoglobulin E and are responsible for host defense and an array of diseases related to immune system. We aimed in this study to characterize the pathways and gene signatures of human cord blood-derived MCs (hCBMCs) in comparison to cells originating from CD34− progenitors using next-generation knowledge discovery methods. CD34+ cells were isolated from human umbilical cord blood using magnetic activated cell sorting and differentiated into MCs with rhIL-6 and rhSCF supplementation for 6–8 weeks. The purity of hCBMCs was analyzed by flow cytometry exhibiting the surface markers CD117+CD34−CD45−CD23−FcεR1αdim. Total RNA from hCBMCs and CD34− cells were isolated and hybridized using microarray. Differentially expressed genes were analyzed using iPathway Guide and Pre-Ranked Gene Set Enrichment Analysis. Next-generation knowledge discovery platforms revealed MC-specific gene signatures and molecular pathways enriched in hCBMCs and pertain the immunological response repertoire.

Published in Experimental and Molecular Pathology

ISSN: 1096-0945 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Pathology
Website: https://www.sciencedirect.com/journal/experimental-and-molecular-pathology

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