Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype

Lin Zhang; Mei Xu; Gang Liu; Rongrong Wu; Shulin Meng; Kang Xiahou; Ning Jiang; Yongxiang Zhang; Wenxia Zhou

Stem Cell Research (Dec 2019)

Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype

Lin Zhang,
Mei Xu,
Gang Liu,
Rongrong Wu,
Shulin Meng,
Kang Xiahou,
Ning Jiang,
Yongxiang Zhang,
Wenxia Zhou

Affiliations

Lin Zhang: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, China
Mei Xu: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China
Gang Liu: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China
Rongrong Wu: Beijing 302 Hospital, Beijing, China
Shulin Meng: IxCell BIOTECHNOLOGY Co.,Ltd, Shanghai, China
Kang Xiahou: IxCell BIOTECHNOLOGY Co.,Ltd, Shanghai, China
Ning Jiang: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; Correspondence authors at: Beijing Institute of Pharmacology and Toxicology; 27 Taiping Road, 100850, Beijing, China.
Yongxiang Zhang: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, China; Correspondence authors at: Beijing Institute of Pharmacology and Toxicology; 27 Taiping Road, 100850, Beijing, China.
Wenxia Zhou: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; Correspondence authors at: Beijing Institute of Pharmacology and Toxicology; 27 Taiping Road, 100850, Beijing, China.

Journal volume & issue: Vol. 41

Abstract

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A 62-year old sporadic Alzheimer's disease (sAD) male patient with APOE3 (ε3/ε3) genotype donated his peripheral blood mononuclear cells (PBMCs). The induced pluripotent stem cell (iPSC) line was established by episomal vector system with the four factors OCT4, SOX2, C-MYC and KLF4. EB differentiation in vitro showed that iPSC line had a potential to differentiate into three germ layers. More than 90% cells expressed NANOG, OCT4 and SSEA4 detected by flow cytometry. In addition, the iPSC line was karyotypically normal. The iPSC line may provide new valuable tools for studying pathogenesis of sAD and screening candidate drugs for the disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal