Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells
Lanlan Zheng,
Fujie Zhao,
Jiaxi Ru,
Lintao Liu,
Zi Wang,
Nianxiang Wang,
Xiangli Shu,
Zhanyong Wei,
Huichen Guo
Affiliations
Lanlan Zheng
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Fujie Zhao
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Jiaxi Ru
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Lintao Liu
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Zi Wang
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Nianxiang Wang
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Xiangli Shu
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Zhanyong Wei
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Huichen Guo
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
A transmissible gastroenteritis virus (TGEV) is a porcine enteropathogenic coronavirus, causing acute swine enteric disease especially in suckling piglets. Mesoporous silica nanoparticles (MSNs) are safe vaccine adjuvant, which could enhance immune responses. Our previous research confirmed that nano silicon had immune-enhancing effects with inactivated TGEV vaccine. In this study, we further clarified the immune-enhancing mechanism of the inactivated TGEV vaccine with MSNs on porcine dendritic cells (DCs). Our results indicated that the inactivated TGEV vaccine with MSNs strongly enhanced the activation of the DCs. Expressions of TLR3, TLR5, TLR7, TLR9, and TLR10, cytokines IFN-α, IL-1β, IL-6, IL-12, and TNF-α, cytokine receptor CCR-7 of immature DCs were characterized and showed themselves to be significantly higher in the inactivated TGEV vaccine with the MSN group. In summary, the inactivated TGEV vaccine with MSNs has effects on the phenotype and function of porcine DCs, which helps to better understand the immune-enhancing mechanism.
