Integrated Deadenylase Genetic Association Network and Transcriptome Analysis in Thoracic Carcinomas
Athanasios Kyritsis,
Eirini Papanastasi,
Ioanna Kokkori,
Panagiotis Maragozidis,
Demetra S. M. Chatzileontiadou,
Paschalina Pallaki,
Maria Labrou,
Sotirios G. Zarogiannis,
George P. Chrousos,
Dimitrios Vlachakis,
Konstantinos I. Gourgoulianis,
Nikolaos A. A. Balatsos
Affiliations
Athanasios Kyritsis
Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 415 00 Larissa, Greece
Eirini Papanastasi
Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 415 00 Larissa, Greece
Ioanna Kokkori
Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, Biopolis, 411 10 Larissa, Greece
Panagiotis Maragozidis
Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 415 00 Larissa, Greece
Demetra S. M. Chatzileontiadou
Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 415 00 Larissa, Greece
Paschalina Pallaki
Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 415 00 Larissa, Greece
Maria Labrou
Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 415 00 Larissa, Greece
Sotirios G. Zarogiannis
Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, Biopolis, 411 10 Larissa, Greece
George P. Chrousos
University Research Institute of Maternal and Child Health and Precision Medicine, ‘Aghia Sophia’ Children’s Hospital, National and Kapodistrian University of Athens, 115 27 Athens, Greece
Dimitrios Vlachakis
University Research Institute of Maternal and Child Health and Precision Medicine, ‘Aghia Sophia’ Children’s Hospital, National and Kapodistrian University of Athens, 115 27 Athens, Greece
Konstantinos I. Gourgoulianis
Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, Biopolis, 411 10 Larissa, Greece
Nikolaos A. A. Balatsos
Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 415 00 Larissa, Greece
The poly(A) tail at the 3′ end of mRNAs determines their stability, translational efficiency, and fate. The shortening of the poly(A) tail, and its efficient removal, triggers the degradation of mRNAs, thus, regulating gene expression. The process is catalyzed by a family of enzymes, known as deadenylases. As the dysregulation of gene expression is a hallmark of cancer, understanding the role of deadenylases has gained additional interest. Herein, the genetic association network shows that CNOT6 and CNOT7 are the most prevalent and most interconnected nodes in the equilibrated diagram. Subsequent silencing and transcriptomic analysis identifies transcripts possibly regulated by specific deadenylases. Furthermore, several gene ontologies are enriched by common deregulated genes. Given the potential concerted action and overlapping functions of deadenylases, we examined the effect of silencing a deadenylase on the remaining ones. Our results suggest that specific deadenylases target unique subsets of mRNAs, whilst at the same time, multiple deadenylases may affect the same mRNAs with overlapping functions.
