PROCEDEU DE OBŢINERE A SE-EXOPOLIZAHARIDELOR PRODUSE DE CIANOBACTERIA SPIRULINA PLATENSIS

Abstract

Au fost obţinute preparate de Se-EPZ şi EPZ din cianobacteria Spirulina platensis cultivată pe mediu nutritiv cu şi fără adaos de selenit. Extractele parţial purificate (după înlăturarea fracţiei proteice) au fost supuse separării prin metoda HPLC pe coloana de Toyo-Pearl HW40F. Au fost scanate spectrele UV ale preparatelor de exopolizaharide şi elaborată schema tehnologică de realizare a unui procedeu de obţinere a Se-EPZ produse de Spirulina platensis. PROCESS FOR OBTAINING OF Se-EXOPOLISAHARIDES PRODUCED BY CYANOBACTERIUM SPIRULINA PLATENSISSelenium-containing (Se-EPS) and EPS from Spirulina platensis cyanobacterium grown on the nutritive medium with and without selenite supplementation were obtained. The partially purified extracts (after removal of the proteins fraction) were subjected to HPLC separation on the Toyo-Pearl HW40F column. The UV spectra of the exopoly­sac­charide preparations have been scanned and the technological scheme for obtaining of Se-EPZ produced by Spirulina platensis has been developed.

Keywords

• exopolysaccharides, selenium, Spirulina platensis, Se accumulation, FPLC.