The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

Aisha Yesbolatova; Yuichiro Saito; Naomi Kitamoto; Hatsune Makino-Itou; Rieko Ajima; Risako Nakano; Hirofumi Nakaoka; Kosuke Fukui; Kanae Gamo; Yusuke Tominari; Haruki Takeuchi; Yumiko Saga; Ken-ichiro Hayashi; Masato T. Kanemaki

doi:10.1038/s41467-020-19532-z

Nature Communications (Nov 2020)

The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

Aisha Yesbolatova,
Yuichiro Saito,
Naomi Kitamoto,
Hatsune Makino-Itou,
Rieko Ajima,
Risako Nakano,
Hirofumi Nakaoka,
Kosuke Fukui,
Kanae Gamo,
Yusuke Tominari,
Haruki Takeuchi,
Yumiko Saga,
Ken-ichiro Hayashi,
Masato T. Kanemaki

Affiliations

Aisha Yesbolatova: Department of Chromosome Science, National Institute of Genetics, Research Organization of Information and Systems (ROIS)
Yuichiro Saito: Department of Chromosome Science, National Institute of Genetics, Research Organization of Information and Systems (ROIS)
Naomi Kitamoto: Department of Chromosome Science, National Institute of Genetics, Research Organization of Information and Systems (ROIS)
Hatsune Makino-Itou: Department of Gene Function and Phenomics, National Institute of Genetics, ROIS
Rieko Ajima: Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI)
Risako Nakano: Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, University of Tokyo
Hirofumi Nakaoka: Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI)
Kosuke Fukui: Department of Biochemistry, Okayama University of Science
Kanae Gamo: FIMECS, Inc.
Yusuke Tominari: FIMECS, Inc.
Haruki Takeuchi: Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, University of Tokyo
Yumiko Saga: Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI)
Ken-ichiro Hayashi: Department of Biochemistry, Okayama University of Science
Masato T. Kanemaki: Department of Chromosome Science, National Institute of Genetics, Research Organization of Information and Systems (ROIS)

DOI: https://doi.org/10.1038/s41467-020-19532-z
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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Auxin-inducible degron systems can be leaky and require high doses of auxin. Here the authors establish AID2 which uses an OsTIR1 mutant and the ligand 5-Ph-IAA to overcome these problems and establish AID-mediated target depletion in mice.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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