Colloquium Agrariae (Jan 2021)

ACETOINE AND CHITINASE PRODUCTION BY NATIVE RIZOBACTERIA FROM WESTERN PARANÁ

  • João Paulo Silva Monteiro,
  • Anderson José Scherer,
  • Lucas Mateus Hass,
  • Luana Patrícia Pinto Korber,
  • Guilherme Peixoto de Freitas,
  • Vivian Carré-Missio,
  • Marco Antônio Bacellar Barreiros,
  • Isac George Rosset,
  • Luciana Grange

Journal volume & issue
Vol. 16, no. 6
pp. 1 – 9

Abstract

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The objective of this study was to investigate 28 strains of native rhizobacteria from the west region of Paraná, evaluating the acetoin and chitinase production capacity, because of their role in biocontrol. To quantify the acetoin production capacity, the isolates were submitted to the modified Voges-Proskauer (VP) assay in Clark & Lubs liquid medium for glucose fermentation. Metabolite production was evaluated in a spectrophotometer and it showed a wide variation in concentrations (0,476 to 1,865) for different isolates. Strains 241, 320, 326, and 273 showed higher values. According to positive results in the qualitative test of VP, 11 isolates were chosen for the tests in minimal medium containing chitin as the only source of carbon. Isolates that presented the degradation halo formation were considered as chitinolytic bacteria, evidencing chitinase activity. Among these strains, two belonged to the genus Bacillus spp. (56 and 121) and two to the genus Enterobacter spp. (292 and 151). Therefore, the present work was able to identify native strains with potential for biocontrol studies allowing to continue investigations of other metabolites produced by those bacteria in this collection regarding plant protection.

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