Identifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry
Bingbing Ren,
Ahmed Sayed,
Hwei Tan,
Yu Mok,
Ee Chen
Affiliations
Bingbing Ren
Department of Biochemistry, National University of Singapore, Yong Loo Lin School of Medicine, Singapore
Ahmed Sayed
Department of Biological Sciences, National University of Singapore, Singapore
Hwei Tan
Department of Biochemistry, National University of Singapore, Yong Loo Lin School of Medicine, SingaporeNational University Health System (NUHS), Singapore
Yu Mok
Department of Biological Sciences, National University of Singapore, Singapore
Ee Chen
Department of Biochemistry, National University of Singapore, Yong Loo Lin School of Medicine, SingaporeNational University Health System (NUHS), Singapore
Histone post-translational modifications (PTMs) regulate numerous cellular processes, including gene transcription, cell division, and DNA damage repair. Most histone PTMs affect the recruitment or exclusion of reader proteins from chromatin. Here, we present a protocol to measure affinity and interaction kinetics between histone peptides and the recombinant protein using Bio-layer interferometry.
