Potential of biological arsenite oxidation in sand filtration units at arsenic–iron removal plants (AIRPs) in Bangladesh

Abstract

Abstract This study is aimed to investigate biological oxidation of arsenic [As(III)] in sand component of arsenic–iron removal plant (AIRP). Water and sand samples were collected from the municipal and household AIRP units (MAIRP and HAIRPs) at Manikganj district, Bangladesh. These samples were analyzed using arsenic speciation cartridge with inductively coupled plasma-mass spectrometry for arsenic and iron detection and phylogenetic analysis based on aoxB gene sequencing. It was observed that arsenic concentration in the raw water, before and after sand filtration, ranged from 13.0–81.0 to 4.0–21.0 μg/L, respectively, which majorly contained reduced arsenic [As(III)] in the inlet and oxidized arsenic [As(V)] in the outlet. The results of our batch experiments showed that indeed sand unit of MAIRP oxidized As(III) with 36 g/h estimated biological oxidation potential which was considered enough to oxidize all the As(III) loading (6.3 g/h) in the influent. Additionally, bacterial arsenite oxidase gene (aoxB) was detected in the sand sample and has 80% sequence similarity with Polymorphum gilvum, an alphaproteobacteria on the phylogenetic tree. This study, therefore, revealed that AIRP sand units have enough potential of biological activity to ensure overall arsenic removal through As(III) oxidation. However, future research is necessary to unfold the basic mechanistic approach explored in this plant for further modification if need arises.

Keywords

• Arsenite
• Bangladesh
• Biological activity
• Sand filtration