Frontiers in Immunology (Mar 2013)

Thymic function recovery after unrelated donor cord blood or T-cell depleted HLA-haploidentical stem cell transplantation correlates with leukemia relapse

  • Emmanuel eClave,
  • Daniela eLisini,
  • Corinne eDouay,
  • Giovana eGiorgiani,
  • Marc eBUSSON,
  • Marco eZecca,
  • Francesca eMoretta,
  • Gloria eAcquafredda,
  • Letizia Pomponia Brescia,
  • Franco eLocatelli,
  • Antoine eToubert

DOI
https://doi.org/10.3389/fimmu.2013.00054
Journal volume & issue
Vol. 4

Abstract

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Use of alternative donors/sources of hematopoietic stem cells (HSC), such as cord blood (CB) or HLA-haploidentical (Haplo) related donors, is associated with a significant delay in immune reconstitution after transplantation. Long-term T-cell immune reconstitution largely relies on the generation of new T cells in the recipient thymus, which can be evaluated through signal joint (sj) and beta T-cell-Receptor Excision Circles (TREC) quantification. We studied two groups of 33 and 24 children receiving, respectively, HSC Transplantation (HSCT) from an HLA-haploidentical family donor or an unrelated CB donor, for both malignant (46) and non-malignant disorders (11). Relative and absolute sj and beta-TREC values indicated comparable thymic function reconstitution at 3 and 6 months after the allograft in both groups. Compared to children with non-malignant disorders, those with hematological malignancies had significantly lower pre-transplantation TREC counts. Patients who relapsed after HSCT had a significantly less efficient thymic function both before and 6 months after HSCT with especially low beta-TREC values, this finding suggesting an impact of early intra-thymic T-cell differentiation on the occurrence of leukemia relapse.

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