Nature Communications (Nov 2021)
Structural dynamics in the water and proton channels of photosystem II during the S2 to S3 transition
- Rana Hussein,
- Mohamed Ibrahim,
- Asmit Bhowmick,
- Philipp S. Simon,
- Ruchira Chatterjee,
- Louise Lassalle,
- Margaret Doyle,
- Isabel Bogacz,
- In-Sik Kim,
- Mun Hon Cheah,
- Sheraz Gul,
- Casper de Lichtenberg,
- Petko Chernev,
- Cindy C. Pham,
- Iris D. Young,
- Sergio Carbajo,
- Franklin D. Fuller,
- Roberto Alonso-Mori,
- Alex Batyuk,
- Kyle D. Sutherlin,
- Aaron S. Brewster,
- Robert Bolotovsky,
- Derek Mendez,
- James M. Holton,
- Nigel W. Moriarty,
- Paul D. Adams,
- Uwe Bergmann,
- Nicholas K. Sauter,
- Holger Dobbek,
- Johannes Messinger,
- Athina Zouni,
- Jan Kern,
- Vittal K. Yachandra,
- Junko Yano
Affiliations
- Rana Hussein
- Institut für Biologie, Humboldt-Universität zu Berlin
- Mohamed Ibrahim
- Institut für Biologie, Humboldt-Universität zu Berlin
- Asmit Bhowmick
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Philipp S. Simon
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Ruchira Chatterjee
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Louise Lassalle
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Margaret Doyle
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Isabel Bogacz
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- In-Sik Kim
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Mun Hon Cheah
- Department of Chemistry - Ångström, Molecular Biomimetics, Uppsala University
- Sheraz Gul
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Casper de Lichtenberg
- Department of Chemistry - Ångström, Molecular Biomimetics, Uppsala University
- Petko Chernev
- Department of Chemistry - Ångström, Molecular Biomimetics, Uppsala University
- Cindy C. Pham
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Iris D. Young
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Sergio Carbajo
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Franklin D. Fuller
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Roberto Alonso-Mori
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Alex Batyuk
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Kyle D. Sutherlin
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Aaron S. Brewster
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Robert Bolotovsky
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Derek Mendez
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- James M. Holton
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Nigel W. Moriarty
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Paul D. Adams
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Uwe Bergmann
- Department of Physics, University of Wisconsin–Madison
- Nicholas K. Sauter
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Holger Dobbek
- Institut für Biologie, Humboldt-Universität zu Berlin
- Johannes Messinger
- Department of Chemistry - Ångström, Molecular Biomimetics, Uppsala University
- Athina Zouni
- Institut für Biologie, Humboldt-Universität zu Berlin
- Jan Kern
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Vittal K. Yachandra
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Junko Yano
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- DOI
- https://doi.org/10.1038/s41467-021-26781-z
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 16
Abstract
The oxygen-evolving complex in Photosystem II (PSII) catalyzes the light-driven oxidation of water to oxygen and it is still under debate how the water reaches the active site. Here, the authors analyse time-resolved XFEL-based crystal structures of PSII that were determined at room temperature and report the structures of the waters in the putative channels surrounding the active site at various time-points during the reaction cycle and conclude that the O1 channel is the likely water intake pathway and the Cl1 channel the likely proton release pathway.
