A comparative study on chemical composition, antibiofilm and biological activities of leaves extracts of four Tunisian olive cultivars
Hayet Edziri,
Raouf Jaziri,
Hechmi Chehab,
Luc Verschaeve,
Guido Flamini,
Dalenda Boujnah,
Mohamed Hammami,
Mahjoub Aouni,
Maha Mastouri
Affiliations
Hayet Edziri
Laboratory of Transmissible Diseases and Biologically Active Substances, Faculty of Pharmacy, 5000, Monastir, Tunisia; Corresponding author.
Raouf Jaziri
Department of Healthcare Services and Hospital Management, University of Jeddah, Saudi Arabia; Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium
Hechmi Chehab
Institut of Olive Sousse, B.P.40 Ibn Khaldoun, 4061, Sousse, Tunisia
Luc Verschaeve
Sciensano, Risk and Health Impact Assessment Service, Brussels, Belgium; Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium
Guido Flamini
Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium; Dipartimento di Farmacia, Via Bonanno 6, 56126, Pisa, Italy; Centro Interdipartimentale di Ricerca “Nutraceutica e Alimentazione per la Salute” (NUTRAFOOD), Università di Pisa, Via del Borghetto 80, 56124, Pisa, Italy
Dalenda Boujnah
Institut of Olive Sousse, B.P.40 Ibn Khaldoun, 4061, Sousse, Tunisia
Mohamed Hammami
Laboratory of Biochemistry, USCR Mass Spectrometry, UR Nutrition and Metabolic Disorders, Faculty of Medicine, University of Monastir, 5000, Monastir, Tunisia
Mahjoub Aouni
Laboratory of Transmissible Diseases and Biologically Active Substances, Faculty of Pharmacy, 5000, Monastir, Tunisia
Maha Mastouri
Laboratory of Transmissible Diseases and Biologically Active Substances, Faculty of Pharmacy, 5000, Monastir, Tunisia
Olea europaea L. is one of the most important fruit trees in Tunisia because of its content of many potentially bioactive compounds. The aim of this study was to evaluate the chemical composition, antibiofilm, antiradical and acethylcholinesterase inhibitory activities from four Tunisian cultivars of Olea europaea L., i.e. ‘Chetoui’, ‘Meski’, ‘Oueslati’ and ‘Jarboui’. By means of standardized methods, total phenols were determined and some of them characterized by HPLC. The total phenols and flavonoids contents were found to be the highest in the leaves of Chetoui cultivar. The Chetoui cultivar exhibited an important antioxidant and anticholinesterasic activity and an important anti-biofilm activity against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Candida albicans, Enterococcus faecalis and Escherichia coli, with percentages of inhibition comprised between 83 and 93% at 2xMIC values. Olive leaves extracts could be used in the control of bacterial biofilms in food and food-related environments.
