Histological study of potassium 2-((4-amino-5-(morpholinomethyl)-4H-1,2,4-triazole-3-yl)thio)acetate (PKR-173) corrective influence on the chickens liver state in the condition of tetracycline hepatitis

Abstract

Despite great scientific progress in the sphere of hepatoprotectors development during recent years, the issues of hepatocytes protection and regeneration is relevant enough. In this aspect derivatives of heterocyclic systems are of great interest, in particular, of 1,2,4-triazole. The aim is histological study of the compound of potassium 2-((4-amino-5-(morpholinomethyl)-4H-1,2,4-triazol-3-yl)thio)acetate (PKR-173) corrective influence on the chickens liver under tetracycline hepatitis condition. Materials and methods. As an object of research potassium 2-((4-amino-5- (morpholinomethyl)-4H-1,2,4-triazol-3-yl)thio) acetate was used which is a water-soluble compound. Histological examination was carried out of the intact chickens liver (intact control); of the chickens after tetracycline administration (control pathology); of the chickens which before the administration of tetracycline were administrated PKR-173 compound or thiotriazoline. All chickens were administrated tetracycline per os in the doze of 500 mg/kg for 5 days. PKR-173 compound and thiotriazoline were administrated per os for 3 days before tetracycline in doze of 150 mg/kg and 50 mg/kg respectively. Results. It has been noted that preventive administration of PKR-173 compound for chickens aged 7–18 days visually prevented the development of nonspecific morphological changes which are characteristic of acute moderately severe drug-induced bird’s liver injury. Compound PKR-173 contributed the organ histostructure normalization, prevented dystrophy manifestations, significantly reduced necrosis of hepatocytes and inflammatory reaction in the perivenular zone of triad system. Comparative drug thiotriazoline prevented hepatocytes dystrophy manifestation, in the vast majority of chickens contributed to hepatic plates structure restoration and inflammatory response reduction. In order to confirm the visual data according to the task, a semi-quantitative assessment of the PKR-173 and thiotriazoline corrective effect on chickens liver parenchyma morphological state was carried out. Conclusions. Analysis of the pathological process quantitative characteristics, which developed in the chicken liver after tetracycline administration and corrective influence of the investigated drugs on it, has shown that the PKR-173 compound significantly reduced the manifestations of all the pathological features detected for testing compared to control pathology. The PKR-173 compound is probably not inferior to comparative drug thiotriazoline by normalizing effect on the organ histostructure restoration and antidystrophic action intensity. The PKR-173 compound significantly outperforms the comparative thiotriazoline for anti-necrotic and anti-inflammatory effects.

Keywords

• 4-triazole hepatitis
• histological techniques
• tetracycline