Protective Efficacy of Lyophilized Vesicular Stomatitis Virus–Based Vaccines in Animal Model

Abstract

We evaluated the in vitro effects of lyophilization for 2 vesicular stomatitis virus–based vaccines by using 3 stabilizing formulations and demonstrated protective immunity of lyophilized/reconstituted vaccine in guinea pigs. Lyophilization increased stability of the vaccines, but specific vesicular stomatitis virus–based vaccines will each require extensive analysis to optimize stabilizing formulations.

Keywords

• vesicular stomatitis virus
• vaccines
• Ebola virus
• Lassa virus
• glycoproteins
• lyophilized