Characterization of the complete chloroplast genome sequence of Pueraria montana var. lobata and its phylogenetic implications

Abstract

Pueraria montana var. lobata is a traditional Chinese herb with various medicinal purposes. In this study, the complete chloroplast genome of the P. montana var. lobata was obtained by using Illumina HiSeq X Ten. The chloroplast genome was 153,411 bp long, which consisted of a large single-copy (LSC) region of 84, 131 bp and a short single-copy (SSC) region of 17,990 bp separated by a pair of inverted repeat (IR) regions of 25,645 bp. The whole chloroplast genome contained 111 gene species including 77 protein-coding genes (PCG), 30 tRNA, and 4 rRNA species, with 19 of them occurring in double copies. Introns were detected in 12 PCG and 6 tRNA species. The overall GC content is 35.4%. Phylogenetic analysis indicated that P. montana var. lobata was relatively close to Pachyrhizus erosus in phylogeny.

Keywords

• pueraria montana var. lobata
• chloroplast genome
• phylogenetic analysis
• herb