Quantification of Trypanosoma cruzi in Tissue and Trypanosoma cruzi Killing Assay
Hisako Kayama,
Shoko Kitada,
Kiyoshi Takeda
Affiliations
Hisako Kayama
Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Osaka, JapanWPI Immunology Frontier Research Center, Osaka University, Osaka, Japan, Core Research for Evolutional Science and Technology, Japan Agency for Medical Research and Development, Tokyo, Japan
Shoko Kitada
Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Osaka, JapanWPI Immunology Frontier Research Center, Osaka University, Osaka, Japan, Core Research for Evolutional Science and Technology, Japan Agency for Medical Research and Development, Tokyo, Japan
Kiyoshi Takeda
Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Osaka, JapanWPI Immunology Frontier Research Center, Osaka University, Osaka, Japan, Core Research for Evolutional Science and Technology, Japan Agency for Medical Research and Development, Tokyo, Japan
Infection with Trypanosoma cruzi causes Chagas disease. The methods provided here allow for the quantification of T. cruzi in the liver, heart, and blood of intraperitoneally-infected mice and analysis of the killing activity of the cells infected with T. cruzi in vitro.
