Qualitative Identification of <em>Roseburia hominis</em> in Faeces Samples Obtained from Patients with Irritable Bowel Syndrome and Healthy Individuals
Paulina Pecyna,
Zuzanna Babalska,
Klaudia Szymendera,
Dorota Nowak Malczewska,
Monika Górna,
Maria Jaworska Marcelina,
Marcin Gabryel,
Dorota Mańkowska-Wierzbicka,
Marian Grzymisławski,
Agnieszka Dobrowolska,
Hanna Tomczak,
Małgorzata Chmielewska,
Marzena Gajęcka
Affiliations
Paulina Pecyna
Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland
Zuzanna Babalska
Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland
Klaudia Szymendera
Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland
Dorota Nowak Malczewska
Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland
Monika Górna
Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland
Maria Jaworska Marcelina
Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland
Marcin Gabryel
Department of Gastroenterology, Dietetics and Internal Diseases, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland
Dorota Mańkowska-Wierzbicka
Department of Gastroenterology, Dietetics and Internal Diseases, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland
Marian Grzymisławski
Department of Gastroenterology, Dietetics and Internal Diseases, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland
Agnieszka Dobrowolska
Department of Gastroenterology, Dietetics and Internal Diseases, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland
Hanna Tomczak
Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland
Małgorzata Chmielewska
Chair and Department of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806 Poznan, Poland
Marzena Gajęcka
Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland
Various products coded by genes recognized in microbiota are involved in many biochemical pathways in thehuman body. Bacteria composition in the gastrointestinal tract may be an important aspect of selected diseases’ pathogenesis, including irritable bowel syndrome (IBS). Traditional research methods based on classical microbiology, using selective media for bacterial growth, have proven to be ineffective. The use of genetic methods allows the identification of unidentified microbiota, including anaerobes. Roseburia hominis is a flagellated gut anaerobic commensal bacterium, producing short-fatty acids. The knowledge about the microbial components of the intestinal ecosystem is still very limited, including Roseburia hominis. This study aimed to identify Roseburia hominis in the faeces samples obtained from IBS patients and healthy individuals, using PCR techniques. The differences between studied groups were observed, and it was concluded that R. hominis may play a role in IBS etiology.
