Antidiabetic, anticholinergic and antioxidant activities of aerial parts of shaggy bindweed (Convulvulus betonicifolia Miller subsp.) – profiling of phenolic compounds by LC-HRMS
Zeynebe Bingol,
Hatice Kızıltaş,
Ahmet C. Gören,
Leyla Polat Kose,
Meryem Topal,
Lokman Durmaz,
Saleh H. Alwasel,
İlhami Gulcin
Affiliations
Zeynebe Bingol
Department of Chemistry, Faculty of Sciences, Atatürk University, Erzurum 25240, Turkey
Hatice Kızıltaş
Vocational School of Health Services, Van Yuzuncu Yil University, Van 65080, Turkey
Ahmet C. Gören
Department of Analytical Chemistry, Faculty of Pharmacy, Bezmialem Vakif University, Istanbul 34093, Turkey; Drug Application and Research Center, Bezmialem Vakif University, Istanbul 34093, Turkey
Leyla Polat Kose
Vocational School, Department of Pharmacy Services, Beykent University, Buyukcekmece, Istanbul 34500, Turkey
Meryem Topal
Vocational School of Health Services, Gumushane University, Gumushane 29000, Turkey
Lokman Durmaz
Department of Medical Services and Technology, Cayirli Vocational School, Erzincan Binali Yildirim University, Cayirli, Erzincan 24500, Turkey
Saleh H. Alwasel
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
İlhami Gulcin
Department of Chemistry, Faculty of Sciences, Atatürk University, Erzurum 25240, Turkey; Corresponding author.
In order to evaluate the antioxidant activity of evaporated ethanolic extract (EESB) and lyophilized water extract (WESB) of Shaggy bindweed (Convulvulus betonicifolia Mill. Subs), some putative antioxidant methods such as DPPH· scavenging activity, ABTS•+ scavenging effect, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+) binding activities were separately performed. Also, ascorbic acid, α-tocopherol and BHT were used as the standard compounds. Additionally, some phenolic compounds that responsible for antioxidant abilities of EESB and WESB were screened by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the same concentration, EESB and WESB demonstrated effective antioxidant abilities when compared to standards. In addition, EESB demonstrated IC50 values of 1.946 μg/mL against acetylcholinesterase (AChE), 0.815 μg/mL against α-glycosidase and 0.675 μg/mL against α-amylase enzymes.
