Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein

Go-Woon Choi; June-Pyo Oh; In-Sook Cho; Hye-Kyoung Ju; Wen-Xing Hu; Boram Kim; Eun-Young Seo; Jong-Seok Park; Leslie L Domier; John Hammond; Kihak Song; Hyoun-Sub Lim

doi:10.5423/PPJ.NT.12.2018.0286

The Plant Pathology Journal (Oct 2019)

Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein

Go-Woon Choi,
June-Pyo Oh,
In-Sook Cho,
Hye-Kyoung Ju,
Wen-Xing Hu,
Boram Kim,
Eun-Young Seo,
Jong-Seok Park,
Leslie L Domier,
John Hammond,
Kihak Song,
Hyoun-Sub Lim

Affiliations

Go-Woon Choi: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
June-Pyo Oh: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
In-Sook Cho: Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Korea
Hye-Kyoung Ju: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
Wen-Xing Hu: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
Boram Kim: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
Eun-Young Seo: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
Jong-Seok Park: Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
Leslie L Domier: Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
John Hammond: United States Department of Agriculture-Agricultural Research Service, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA
Kihak Song: Department of Urology, Chungnam National University School of Medicine, Daejeon 34134, Korea
Hyoun-Sub Lim: Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea

DOI: https://doi.org/10.5423/PPJ.NT.12.2018.0286
Journal volume & issue: Vol. 35, no. 5
pp. 538 – 542

Abstract

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In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.

Published in The Plant Pathology Journal

ISSN: 1598-2254 (Print); 2093-9280 (Online)
Publisher: Hanrimwon Publishing Company
Country of publisher: Korea, Republic of
LCC subjects: Agriculture: Plant culture
Website: http://www.ppjonline.org/main.html

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