Cryo-electron tomography provides topological insights into mutant huntingtin exon 1 and polyQ aggregates

Jesús G. Galaz-Montoya; Sarah H. Shahmoradian; Koning Shen; Judith Frydman; Wah Chiu

doi:10.1038/s42003-021-02360-2

Communications Biology (Jul 2021)

Cryo-electron tomography provides topological insights into mutant huntingtin exon 1 and polyQ aggregates

Jesús G. Galaz-Montoya,
Sarah H. Shahmoradian,
Koning Shen,
Judith Frydman,
Wah Chiu

Affiliations

Jesús G. Galaz-Montoya: Department of Bioengineering and James H. Clark Center, Stanford University
Sarah H. Shahmoradian: Department of Biology and Chemistry, Laboratory of Biomolecular Research, Paul Scherrer Institute
Koning Shen: Department of Biology, Stanford University
Judith Frydman: Department of Biology, Stanford University
Wah Chiu: Department of Bioengineering and James H. Clark Center, Stanford University

DOI: https://doi.org/10.1038/s42003-021-02360-2
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 9

Abstract

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Galaz-Montoya et al. report nanometer-resolution 3D cryo-electron tomography structures of mutant huntingtin (mHTT) and polyglutamine-only (polyQ) filaments in large aggregates free of stains, fixatives, tags, or dehydration artifacts. These results provide a framework for future structural studies of mHTT and polyQ aggregates, thereby improving our understanding of polyQ disorders such as Huntington disease.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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