Nature Communications (Dec 2016)
Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells
- Henning Kempf,
- Ruth Olmer,
- Alexandra Haase,
- Annika Franke,
- Emiliano Bolesani,
- Kristin Schwanke,
- Diana Robles-Diaz,
- Michelle Coffee,
- Gudrun Göhring,
- Gerald Dräger,
- Oliver Pötz,
- Thomas Joos,
- Erik Martinez-Hackert,
- Axel Haverich,
- Falk F. R. Buettner,
- Ulrich Martin,
- Robert Zweigerdt
Affiliations
- Henning Kempf
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- Ruth Olmer
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- Alexandra Haase
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- Annika Franke
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- Emiliano Bolesani
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- Kristin Schwanke
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- Diana Robles-Diaz
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- Michelle Coffee
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- Gudrun Göhring
- REBIRTH-Cluster of Excellence, Hannover Medical School
- Gerald Dräger
- REBIRTH-Cluster of Excellence, Hannover Medical School
- Oliver Pötz
- Natural and Medical Sciences Institute at the University of Tuebingen (NMI)
- Thomas Joos
- Natural and Medical Sciences Institute at the University of Tuebingen (NMI)
- Erik Martinez-Hackert
- Department of Biochemistry and Molecular Biology, Michigan State University
- Axel Haverich
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- Falk F. R. Buettner
- REBIRTH-Cluster of Excellence, Hannover Medical School
- Ulrich Martin
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- Robert Zweigerdt
- Department of Cardiothoracic, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Transplantation and Vascular Surgery (HTTG), Hannover Medical School
- DOI
- https://doi.org/10.1038/ncomms13602
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 13
Abstract
Differentiation of human pluripotent stem cells (hPSC) mimics aspects of embryonic developmentin vitrobut is poorly understood. Here, the authors identify bulk cell density as a key parameter directing transition from pluripotency to primitive streak-like priming in hPSCs via secreted LEFTY/CERBERUS.
