Investigating the K+ sensitivity of cellular metabolism by extracellular flux analysis

Abstract

Summary: We have recently demonstrated that the activity of hexokinase 2 is dependent on the intracellular potassium ion (K+) concentration ([K+]). To analyze the K+ dependency of the cell metabolism in cell populations, we used an extracellular flux analyzer to assess oxygen consumption and acidification rates as well-established measures of oxidative- and glycolytic metabolic activities. This protocol describes in detail how a potential K+ sensitivity of the cell metabolism can be elucidated by extracellular flux analysis.For complete details on the use and execution of this protocol, please refer to Bischof et al. (2021).

Keywords

• Cell Biology
• Cell-based Assays
• Cancer
• High Throughput Screening
• Metabolism
• Molecular Biology