Nanobody-enabled monitoring of kappa opioid receptor states

Tao Che; Justin English; Brian E. Krumm; Kuglae Kim; Els Pardon; Reid H. J. Olsen; Sheng Wang; Shicheng Zhang; Jeffrey F. Diberto; Noah Sciaky; F. Ivy Carroll; Jan Steyaert; Daniel Wacker; Bryan L. Roth

doi:10.1038/s41467-020-14889-7

Nature Communications (Mar 2020)

Nanobody-enabled monitoring of kappa opioid receptor states

Tao Che,
Justin English,
Brian E. Krumm,
Kuglae Kim,
Els Pardon,
Reid H. J. Olsen,
Sheng Wang,
Shicheng Zhang,
Jeffrey F. Diberto,
Noah Sciaky,
F. Ivy Carroll,
Jan Steyaert,
Daniel Wacker,
Bryan L. Roth

Affiliations

Tao Che: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine
Justin English: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine
Brian E. Krumm: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine
Kuglae Kim: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine
Els Pardon: Structural Biology Brussels, Vrije Universiteit Brussel (VUB)
Reid H. J. Olsen: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine
Sheng Wang: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine
Shicheng Zhang: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine
Jeffrey F. Diberto: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine
Noah Sciaky: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine
F. Ivy Carroll: Research Triangle Institute, Research Triangle Park
Jan Steyaert: Structural Biology Brussels, Vrije Universiteit Brussel (VUB)
Daniel Wacker: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine
Bryan L. Roth: Department of Pharmacology, University of North Carolina at Chapel Hill School of Medicine

DOI: https://doi.org/10.1038/s41467-020-14889-7
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 12

Abstract

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Recent studies revealed that G protein-coupled receptors rapidly interconvert between multiple states. Here, authors use the kappa opioid receptor (KOR) and show how two state-dependent nanobodies provide real-time reporting of ligand stabilized states with KOR and other GPCRs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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