Biological Research (Nov 2018)

Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)

  • Rolando Garcia-Gonzales,
  • José Pico-Mendoza,
  • Karla Quiroz,
  • Basilio Carrasco,
  • Pablo Cáceres,
  • Borys Chong-Perez,
  • Hugo Pino,
  • Marjorie Seiltgens,
  • Eglis Greck,
  • Peter D. S. Caligari

DOI
https://doi.org/10.1186/s40659-018-0193-4
Journal volume & issue
Vol. 51, no. 1
pp. 1 – 4

Abstract

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Abstract Background Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. Results Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. Conclusions From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.

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