Cryopreservation Induces Alterations of miRNA and mRNA Fragment Profiles of Bull Sperm

Aishao Shangguan; Hao Zhou; Wei Sun; Rui Ding; Xihe Li; Jiajia Liu; Yang Zhou; Xing Chen; Fengling Ding; Liguo Yang; Shujun Zhang

doi:10.3389/fgene.2020.00419

Frontiers in Genetics (May 2020)

Cryopreservation Induces Alterations of miRNA and mRNA Fragment Profiles of Bull Sperm

Aishao Shangguan,
Hao Zhou,
Wei Sun,
Rui Ding,
Xihe Li,
Jiajia Liu,
Yang Zhou,
Xing Chen,
Fengling Ding,
Liguo Yang,
Shujun Zhang

Affiliations

Aishao Shangguan: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China
Hao Zhou: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China
Wei Sun: Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, China
Rui Ding: Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, China
Xihe Li: Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, China
Jiajia Liu: School of Biological Science and Technology, University of Jinan, Jinan, China
Yang Zhou: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China
Xing Chen: Institute of Animal Husbandry and Veterinary, Wuhan Academy of Agricultural Science, Wuhan, China
Fengling Ding: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China
Liguo Yang: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China
Shujun Zhang: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China

DOI: https://doi.org/10.3389/fgene.2020.00419
Journal volume & issue: Vol. 11

Abstract

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Although cryopreservation of bull semen is widely used commercially in the livestock breeding industry, cryopreservation results in low fertility of bull sperm. As an important regulatory factor, the alteration of small non-coding RNA (sncRNA) profile during cryopreservation of bull sperm is not yet completely known. In the present study, we sequenced sncRNAs of frozen and fresh sperm to study the link of alteration of the sncRNA profiles (particularly in miRNAs and mRNA fragments) with low sperm fertility caused by cryopreservation. We identified 55 miRNAs and 526 mRNA fragments differentially expressed (DE) between frozen and fresh sperm. Subsequently, the functional analysis revealed that targeted genes of DE miRNAs in sperm had roles in the fertilization, ATP, and apoptosis. Instead, targeted genes of DE miRNAs in cow metaphase II oocyte were significantly enriched in the MAPK signaling pathway, autophagy-animal pathway, and mitophagy-animal pathway. Additionally, biological processes of DNA repair, spermatid development, response to temperature stimulus, and cellular response to DNA damage stimulus were enriched by mRNA fragments. In conclusion, we found that DE miRNAs or DE mRNA fragments in cryopreservation may influence the fertility of sperm, these findings will provide the reference to improve the cryopreservation technology of bull semen.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

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