EFFECTS OF CURCUMIN AND LUTEOLIN ON VIABILITY AND CELL DEATH INDUCTION IN NFS-60 CELL LINE

Abstract

Inducing cell death in tumor cells has been recognized as a promising strategy in curing tumors. Concurrently, interest has been rising for demanding and extensive clinical trials based on the effects of natural products, especially those with long-known usage in folk medicine. Aiming to contribute to the overall knowledge of antitumour potential of curcumin and luteolin, we analyzed the effect of their concentration gradient (5, 10 and 20 µM) on cell death induction in NFS-60 cell line, using Trypan blue exclusion assay and TransDetect® Annexin V-EGFP/PI assay. The results show that both tested agents induce cell death, especially in higher concentrations, but further investigations are needed to elucidate the mechanisms behind it.

Keywords

• Bioflavonoids
• apoptosis
• necrosis
• cytotoxicity
• murine leukemia