Identification and functional comparison of novel alternatively spliced isoforms of human YAP
Lianlian Liu,
Junlei Zhang,
Jiaqi Wang,
Yanping Tian,
Jiali Wang,
Yixiao Xu,
Yuda Cheng,
Meng Yu,
Jiangjun Wang,
Yi Yang,
Xueyue Wang,
Ran Yang,
Wei Wu,
Chen Zhang,
Yan Hu,
Rui Jian,
Lan Xiao,
Yan Ruan
Affiliations
Lianlian Liu
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Junlei Zhang
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Jiaqi Wang
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Yanping Tian
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Jiali Wang
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Yixiao Xu
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Yuda Cheng
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Meng Yu
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Jiangjun Wang
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Yi Yang
Experimental Center of Basic Medicine, College of Basic Medical Sciences Army Medical University Chongqing China
Xueyue Wang
Department of Pediatrics The General Hospital of PLA Tibet Military Area Command Lhasa China
Ran Yang
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Wei Wu
Thoracic Surgery Department Southwest Hospital, The First Hospital Affiliated to Army Medical University Chongqing China
Chen Zhang
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Yan Hu
Department of Military Basic Training and Army Management, Army Health Service Training Base Army Medical University Chongqing China
Rui Jian
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
Lan Xiao
Department of Histology and Embryology, Chongqing Key Laboratory of Neurobiology, Brain and Intelligence Research Key Laboratory of Chongqing Education Commission Army Medical University Chongqing China
Yan Ruan
Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology Army Medical University Chongqing China
As a key effector of the Hippo pathway, yes‐associated protein (YAP) is a major regulator of cell proliferation and apoptosis. In this study, 23 hYAP isoforms were identified in HEK293 cells, with 14 isoforms being reported for the first time. These isoforms were classified into hYAP‐a and hYAP‐b isoforms based on the variation in exon 1. The two groups of isoforms showed distinctly different subcellular localizations. hYAP‐a isoforms could activate TEAD‐ or P73‐mediated transcription, affect the proliferation rate, and enhance the cellular chemosensitivity of HEK293 cells. Moreover, different activation abilities and pro‐cytotoxic effects were observed among hYAP‐a isoforms. However, hYAP‐b isoforms were not found to exert any significant biological effects. Our findings add to the knowledge of YAP gene structure and protein‐coding capacity and will help in the elucidation of the function and related molecular mechanisms of the Hippo‐YAP signaling pathway.