Nature Communications (Dec 2019)

Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes

  • Derek Thibault,
  • Paul A. Jensen,
  • Stephen Wood,
  • Christine Qabar,
  • Stacie Clark,
  • Mara G. Shainheit,
  • Ralph R. Isberg,
  • Tim van Opijnen

DOI
https://doi.org/10.1038/s41467-019-13719-9
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 13

Abstract

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Culturing transposon-mutant libraries in pools can mask complex phenotypes. Here the authors present microfluidics mediated droplet Tn-Seq, which encapsulates individual mutants, promotes isolated growth and enables cell-cell interaction analyses.