Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing

Zijun Huo; Jian Tu; An Xu; Yan Li; Donghui Wang; Mo Liu; Ruoji Zhou; Dandan Zhu; Yu Lin; Julian A. Gingold; Chia-Jui Yen; Haipeng Xiao; Ruiying Zhao

Stem Cell Research (Jan 2019)

Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing

Zijun Huo,
Jian Tu,
An Xu,
Yan Li,
Donghui Wang,
Mo Liu,
Ruoji Zhou,
Dandan Zhu,
Yu Lin,
Julian A. Gingold,
Chia-Jui Yen,
Haipeng Xiao,
Ruiying Zhao

Affiliations

Zijun Huo: Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Department of Endocrinology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, PR China
Jian Tu: Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, PR China
An Xu: Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA
Yan Li: Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, PR China
Donghui Wang: Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, PR China
Mo Liu: Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA
Ruoji Zhou: Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas, MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA
Dandan Zhu: Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA
Yu Lin: Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Guangdong Research Center of Metabolic Diseases of Integrated Western and Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, PR China
Julian A. Gingold: Women's Health Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
Chia-Jui Yen: Division of Hematology and Oncology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan
Haipeng Xiao: Department of Endocrinology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, PR China; Corresponding authors.
Ruiying Zhao: Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Corresponding authors.

Journal volume & issue: Vol. 34

Abstract

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Abstract: As one of the most essential genome guardians, p53 and its mutants have been suggested associated with many types of cancers. Many p53 mutants function induce unique phenotypes, including carcinogenesis, metastasis, and drug resistance. The p53(R249S) mutation is the most prevalent and specific mutation associated with liver cancer development. Here, we demonstrate the generation of a heterozygous p53(R249S) mutation in the H9 human embryonic stem cell line using TALEN-mediated genome editing. The generated cell line maintains a normal karyotype, a pluripotent state and the in vivo capacity to develop a teratoma containing all three germ layer tissues.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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