In depth analysis of the Sox4 gene locus that consists of sense and natural antisense transcripts
King-Hwa Ling,
Peter J. Brautigan,
Sarah Moore,
Rachel Fraser,
Melody Pui-Yee Leong,
Jia-Wen Leong,
Shahidee Zainal Abidin,
Han-Chung Lee,
Pike-See Cheah,
Joy M. Raison,
Milena Babic,
Young Kyung Lee,
Tasman Daish,
Deidre M. Mattiske,
Jeffrey R. Mann,
David L. Adelson,
Paul Q. Thomas,
Christopher N. Hahn,
Hamish S. Scott
Affiliations
King-Hwa Ling
Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia; School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA 5005, Australia; NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia; Corresponding author at: NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor. Tel.: +603 8947 2564; fax: +603 8947 2646.
Peter J. Brautigan
Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia
Sarah Moore
Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia
Rachel Fraser
Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia
Melody Pui-Yee Leong
NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia
Jia-Wen Leong
NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia
Shahidee Zainal Abidin
NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia
Han-Chung Lee
NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia
Pike-See Cheah
NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia; School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia; Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor DE, Malaysia
Joy M. Raison
School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia
Milena Babic
Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia
Young Kyung Lee
Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia
Tasman Daish
School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia
Deidre M. Mattiske
fTheme of Laboratory and Community Genetics, Murdoch Childrens Research Institute, Royal Children׳s Hospital, Flemington Road, Parkville, VIC 3052, Australia
Jeffrey R. Mann
gBiomedicine Discovery Institute, Monash University, VIC 3800, Australia
David L. Adelson
School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia
Paul Q. Thomas
School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia
Christopher N. Hahn
Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia
Hamish S. Scott
Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia; School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA 5005, Australia; Corresponding author at: School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA 5005, Australia. Tel.: +618 8222 3651; fax: +618 8222 3146.
SRY (Sex Determining Region Y)-Box 4 or Sox4 is an important regulator of the pan-neuronal gene expression during post-mitotic cell differentiation within the mammalian brain. Sox4 gene locus has been previously characterized with multiple sense and overlapping natural antisense transcripts [1,2]. Here we provide accompanying data on various analyses performed and described in Ling et al. [2]. The data include a detail description of various features found at Sox4 gene locus, additional experimental data derived from RNA-Fluorescence in situ Hybridization (RNA-FISH), Western blotting, strand-specific reverse-transcription quantitative polymerase chain reaction (RT-qPCR), gain-of-function and in situ hybridization (ISH) experiments. All the additional data provided here support the existence of an endogenous small interfering- or PIWI interacting-like small RNA known as Sox4_sir3, which origin was found within the overlapping region consisting of a sense and a natural antisense transcript known as Sox4ot1. Keywords: Endogenous siRNA, Brain development, Natural antisense transcripts
