Identification and validation of stable and novel quantitative trait loci for pod shattering in soybean [Glycinemax (L.) Merr.]

Jia JIA; Huan WANG; Zhan-dong CAI; Ru-qian WEI; Jing-hua HUANG; Qiu-ju XIA; Xiao-hui XIAO; Qi-bin MA; Hai NIAN; Yan-bo CHENG

Journal of Integrative Agriculture (Jan 2022)

Identification and validation of stable and novel quantitative trait loci for pod shattering in soybean [Glycinemax (L.) Merr.]

Jia JIA,
Huan WANG,
Zhan-dong CAI,
Ru-qian WEI,
Jing-hua HUANG,
Qiu-ju XIA,
Xiao-hui XIAO,
Qi-bin MA,
Hai NIAN,
Yan-bo CHENG

Affiliations

Jia JIA: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, P.R.China; The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China; Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou 510642, P.R.China
Huan WANG: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, P.R.China; The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China; Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou 510642, P.R.China
Zhan-dong CAI: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, P.R.China; The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China; Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou 510642, P.R.China
Ru-qian WEI: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, P.R.China; The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China; Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou 510642, P.R.China
Jing-hua HUANG: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, P.R.China; The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China; Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou 510642, P.R.China
Qiu-ju XIA: Beijing Genomics Institute (BGI) Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, P.R.China
Xiao-hui XIAO: The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China
Qi-bin MA: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, P.R.China; The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China; Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou 510642, P.R.China
Hai NIAN: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, P.R.China; The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China; Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou 510642, P.R.China; Correspondence NIAN Hai
Yan-bo CHENG: The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, P.R.China; The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China; Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou 510642, P.R.China; Correspondence CHENG Yan-bo

Journal volume & issue: Vol. 21, no. 11
pp. 3169 – 3184

Abstract

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Pod shattering is an important domesticated trait which can cause great economic loss of crop yield in cultivated soybean. In this study, we utilized two recombinant inbred line populations (RILs, CY, Huachun 2×Wayao; GB, Guizao 1× B13) to identify quantitative trait loci (QTLs) associated with pod shattering in soybean across multiple environments. A total of 14 QTLs for pod shattering were identified in the two RIL populations, which had LOD scores ranging from 2.64 to 44.33 with phenotypic variance explanation (PVE) ranging from 1.33 to 50.85%. One QTL qPS16-1, located on chromosome 16, included a well-known functional gene Poddehiscence1 (Pdh1) that was reported previously. Ten new putative QTLs were validated in two RIL populations, and their LOD scores were between 2.55 and 4.24, explaining 1.33 to 2.60% of the phenotypic variation. Of which four novel QTLs (qPS01-1, qPS03-2, qPS05-1, and qPS07-1) could be detected in two environments where nine genes had specific changes in gene expression. Although the nine genes may have significant effects on pod shattering of soybean, their detailed functions still need to be further explored in the future. The results of this study will facilitate a better understanding of the genetic basis of the pod shattering-resistant trait and benefit soybean molecular breeding for improving pod shattering resistance.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

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