OncoTargets and Therapy (Jul 2020)
miR-195 Serves as a Tumor Suppressor in the Progression of Liposarcoma by Targeting OSBP
Abstract
Ye Cao,1,* Lei Li,1,* Lu Han,2 Jiajia Zheng,3 Chentao Lv1 1Department of General Surgery, Shanghai Public Health Clinical Center, Fudan University, Shanghai, People’s Republic of China; 2Department of General Surgery, Shanghai Jiangong Hospital, Shanghai, People’s Republic of China; 3Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jiajia ZhengDepartment of General Surgery, Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai 200032, People’s Republic of ChinaTel +86-21-64041990Email [email protected] LvDepartment of General Surgery, Shanghai Public Health Clinical Center, Fudan University, No. 2901, Caolang Highway, Jinshan District, Shanghai 200083, People’s Republic of ChinaTel +86-21-37990333Email [email protected]: Liposarcoma was considered as a soft tissue kind of sarcoma with one-fifth in the sarcoma cases of adults. The aim of this study was to explore the role and the potential mechanisms of miR-195 in liposarcoma.Methods: Quantitative real-time PCR (qRT-PCR) was conducted to measure the expression of microRNA-195 (miR-195) and oxysterol-binding protein (OSBP) in liposarcoma. Cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Cell migration was measured by wound healing and transwell assays. Cell cycle phases and apoptosis were examined using flow cytometry analysis. Caspase-3 activity was detected by commercial kit. Binding sites between miR-195 and OSBP were predicted through bioinformatics analysis and confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP). Western blot was used to analyze OSBP level. Xenograft tumor assays were performed to observe the effect of miR-195 overexpression on tumor growth in vivo.Results: miR-195 expression was decreased, whereas OSBP was increased in liposarcoma tissues and cells. Besides, miR-195 upregulation suppressed the proliferative and migrative abilities and induced inhibition on cell growth and promotion on apoptosis in SW872 and 93T449 cells. Mechanically, miR-195 functioned as a suppressor by regulating OSBP expression. Furthermore, OSBP overexpression inverted the effects of miR-195 on cell growth, migration and apoptosis in SW872 and 93T449 cells. miR-195 overexpression also suppressed tumor growth in vivo.Conclusion: miR-195 suppressed cell growth, migration and elevated cell apoptosis via OSBP in liposarcoma.Keywords: liposarcoma, miR-195, OSBP, proliferation, migration, cell cycle, apoptosis
