OncoTargets and Therapy (Jan 2020)
LncRNA-ATB Promotes the Tumorigenesis of Ovarian Cancer via Targeting miR-204-3p
Abstract
Donglan Yuan, 1,* Hua Qian, 1,* Ting Guo, 2 Jun Ye, 2 Chunyan Jin, 2 Xia Liu, 1 Li Jiang, 1 Xiaoxiang Wang, 1 Mei Lin, 3 Hong Yu 4 1Department of Obstetrics and Gynecology, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China; 2Center for Molecular Medicine, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China; 3Scientific Research Office, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China; 4Department of Pathology, Taizhou People’s Hospital, Taizhou, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hong YuDepartment of Pathology, Taizhou People’s Hospital, 399 Hailing Road, Taizhou, Jiangsu 225300, People’s Republic of ChinaEmail [email protected]: Ovarian cancer ranks fifth among the most prevalent cancer type in females all over the world. It is the second most frequent malignant tumor which accounts for 3% of cancer in females. Therefore, to explore the mechanism of carcinogenesis in ovarian cancer is important to develop new treatment methods. It has been previously found that lncRNA-ATB could promote the tumorigenesis of malignant tumors. However, the role of lncRNA-ATB during the progression of ovarian cancer remains unclear.Methods: Gene expressions in tissues or cells were detected by using qRT-PCR. Western blot was performed to investigate the protein expressions in ovarian cancer cells. Cell apoptosis was tested by flow cytometry. Moreover, the correction between lncRNA-ATB and miR-204-3p was examined by Dual-luciferase reporter assay and RNA pulldown. Cell proliferation and invasion were detected by CCK-8, Ki-67 staining and transwell assay, respectively. Finally, xenograft mice model was established to confirm the result of in vitro experiments.Results: LncRNA-ATB silencing significantly inhibited the proliferation and induced apoptosis of ovarian cancer cells. In addition, luciferase activity suggested that lncRNA-ATB negatively regulated miR-204-3p in ovarian cancer. Besides, Nidogen 1 (NID1) was the direct target of miR-204-3p. Overexpression of NID1 could notably reverse the inhibitory effect of lncRNA-ATB knockdown on the progression of ovarian cancer. Finally, lncRNA-ATB silencing notably attenuated the severity of ovarian cancer in vivo.Conclusion: Downregulation of lncRNA-ATB significantly inhibited the tumorigenesis of ovarian cancer in vitro and in vivo, which may serve as a potential novel target for the treatment of ovarian cancer.Keywords: ovarian cancer, lncRNA-ATB, miR-204-3p, NID1
