Specificity of an Enzyme-1 Inked Immunosorbent Assay for Dog Ige Antibody to Japanese Cedar (Cryptomeria Japonica) Pollen

Abstract

We developed a fluorometric enzyme-linked immunosorbent assay (ELISA) for allergen-specific IgE in dogs with the use of monoclonal anti-dog IgE; we assayed IgE antibody to Japanese cedar pollen in the sera of dogs with Japanese cedar pollinosis. To assess the specificity of this ELISA, a pooled serum sample from pollinosis dogs was subjected to gel chromatography. The peak of anti-pollen allergen IgE activity was different from the peaks of total IgA, IgG and IgM. When IgE antibody positive serum was heated at 56°C for 4 h, antibody activity was markedly reduced. Furthermore, polyclonal anti-dog IgA, IgG and IgM did not interfere with anti-pollen allergen IgE activity in the ELISA. From these results, this assay is considered to have a high specificity for dog IgE.

Keywords

• Allergen
• Dog
• IgE
• pollen