Generation of iPSC lines from primary human amniotic fluid cells

Katharina Drews; Peggy Matz; James Adjaye

doi:10.1016/j.scr.2015.11.003

Stem Cell Research (Nov 2015)

Generation of iPSC lines from primary human amniotic fluid cells

Katharina Drews,
Peggy Matz,
James Adjaye

Affiliations

Katharina Drews: Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany
Peggy Matz: Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany
James Adjaye: Max Planck Institute for Molecular Genetics, 14195, Berlin, Germany

DOI: https://doi.org/10.1016/j.scr.2015.11.003
Journal volume & issue: Vol. 15, no. 3
pp. 712 – 714

Abstract

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By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human embryonic stem cell lines (H1 and H9) revealed a Pearson correlation of 0.953 and 0.941 respectively.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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