Simultaneous Determination of Gallic Acid and Ascorbic Acid Using First Derivative Zero-Crossing Spectrophotometric Technique

Abstract

A new spectrophotometric method was used for simultaneous determination of gallic acid and ascorbic acid depending on the first derivative zero-crossing technique. The first derivative spectra of these compounds permitted simultaneous determination of gallic acid and ascorbic acid over the concentration ranges of (1.0 – 30.0) μg/mL and (3.0 – 35.0) μg/mL with detection limits of 0.559 and 0.991 for gallic acid and ascorbic acid, respectively, by techniques of measuring the amplitude of peak-to-base line and zero-crossing at certain wavelengths. The proposed method showed reasonable precision and accuracy and have been applied to determine gallic acid and ascorbic acid in samples of Hibiscus sabdariffa L.

Keywords

• Hibiscus sabdariffa L
• Gallic acid
• Ascorbic acid
• Derivative spectrophotometry.