Exosome-mediated aptamer S58 reduces fibrosis in a rat glaucoma filtration surgery model

Qian-Yi Lin; Xiang-Ji Li; Yu Leng; Xiao-Min Zhu; Min Tang; Yi Lin; Wang-Du Luo; Bing-Cai Jiang; Xia Chen; Lin Xie

doi:10.18240/ijo.2022.05.02

International Journal of Ophthalmology (May 2022)

Exosome-mediated aptamer S58 reduces fibrosis in a rat glaucoma filtration surgery model

Qian-Yi Lin,
Xiang-Ji Li,
Yu Leng,
Xiao-Min Zhu,
Min Tang,
Yi Lin,
Wang-Du Luo,
Bing-Cai Jiang,
Xia Chen,
Lin Xie

Affiliations

Qian-Yi Lin: Lin Xie. Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, 1 Shuanghu Road, Chongqing 401120, China. [email protected]
Xiang-Ji Li: Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China
Yu Leng: Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China
Xiao-Min Zhu: Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China
Min Tang: Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China
Yi Lin: Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China
Wang-Du Luo: Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China
Bing-Cai Jiang: Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China
Xia Chen: Department of Ophthalmology, Daping Hospital of Army Medical University, Chongqing 400042, China
Lin Xie: Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China

DOI: https://doi.org/10.18240/ijo.2022.05.02
Journal volume & issue: Vol. 15, no. 5
pp. 690 – 700

Abstract

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AIM: To confirm whether exosome-mediated delivery of aptamer S58 (Exo-S58) has a better antifibrotic effect than naked S58 in human conjunctival fibroblasts (HConFs) and a rat glaucoma filtration surgery (GFS) model. METHODS: To enhance the effective reaction time of aptamer S58 in vivo, we loaded aptamer S58 into exosomes derived from HEK293T cells by PEI transfection to determine the effect of Exo-S58 in HConFs and a rat GFS model. RESULTS: Exo-S58 can significantly reduce cell proliferation, migration and fibrosis in TGF-β2-induced HConFs. In an in vivo experiment, Exo-S58 treatment prolonged filtering bleb retention and reduced fibrosis compared with naked S58 treatment in GFS rats. CONCLUSION: The exosomes are safe and valid carriers to deliver aptamers. Furthermore, Exo-S58 exhibited superior antifibrotic effect than naked S58 both in HConFs cells and rat GFS models.

Published in International Journal of Ophthalmology

ISSN: 2222-3959 (Print); 2227-4898 (Online)
Publisher: Press of International Journal of Ophthalmology (IJO PRESS)
Country of publisher: China
LCC subjects: Medicine: Ophthalmology
Website: http://www.ijo.cn/gjyken/ch/index.aspx

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