Photothermally Controlled Methotrexate Release System Using β-Cyclodextrin and Gold Nanoparticles
Nataly Silva,
Ana Riveros,
Nicolás Yutronic,
Erika Lang,
Boris Chornik,
Simón Guerrero,
Josep Samitier,
Paul Jara,
Marcelo J. Kogan
Affiliations
Nataly Silva
Department of Chemistry, University of Chile, Las Palmeras 3425, 7800003 Santiago, Chile
Ana Riveros
Department of Pharmacological and Toxicological Chemistry, University of Chile, Sergio Livingston 1007, 8380492 Santiago, Chile
Nicolás Yutronic
Department of Chemistry, University of Chile, Las Palmeras 3425, 7800003 Santiago, Chile
Erika Lang
Department of Biology, CEM, University of Chile, Las Palmeras 3425, 7800003 Santiago, Chile
Boris Chornik
Department of Physics, University of Chile, Beauchef 850, 8370448 Santiago, Chile
Simón Guerrero
Department of Pharmacological and Toxicological Chemistry, University of Chile, Sergio Livingston 1007, 8380492 Santiago, Chile
Josep Samitier
Nanobioengineering Laboratory, Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST) Baldiri Reixac, 10–12, 08028 Barcelona, Spain
Paul Jara
Department of Chemistry, University of Chile, Las Palmeras 3425, 7800003 Santiago, Chile
Marcelo J. Kogan
Department of Pharmacological and Toxicological Chemistry, University of Chile, Sergio Livingston 1007, 8380492 Santiago, Chile
The inclusion compound (IC) of cyclodextrin (CD) containing the antitumor drug Methotrexate (MTX) as a guest molecule was obtained to increase the solubility of MTX and decrease its inherent toxic effects in nonspecific cells. The IC was conjugated with gold nanoparticles (AuNPs), obtained by a chemical method, creating a ternary intelligent delivery system for MTX molecules, based on the plasmonic properties of the AuNPs. Irradiation of the ternary system, with a laser wavelength tunable with the corresponding surface plasmon of AuNPs, causes local energy dissipation, producing the controlled release of the guest from CD cavities. Finally, cell viability was evaluated using MTS assays for β-CD/MTX and AuNPs + β-CD/MTX samples, with and without irradiation, against HeLa tumor cells. The irradiated sample of the ternary system AuNPs + β-CD/MTX produced a diminution in cell viability attributed to the photothermal release of MTX.