The multi-level regulation of clownfish metamorphosis by thyroid hormones
Natacha Roux,
Saori Miura,
Mélanie Dussenne,
Yuki Tara,
Shu-hua Lee,
Simon de Bernard,
Mathieu Reynaud,
Pauline Salis,
Agneesh Barua,
Abdelhay Boulahtouf,
Patrick Balaguer,
Karine Gauthier,
David Lecchini,
Yann Gibert,
Laurence Besseau,
Vincent Laudet
Affiliations
Natacha Roux
Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, Observatoire Océanologique, 66650 Banyuls-sur-Mer, France; Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna son, Okinawa 904-0495, Japan
Saori Miura
Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna son, Okinawa 904-0495, Japan
Mélanie Dussenne
Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, Observatoire Océanologique, 66650 Banyuls-sur-Mer, France
Yuki Tara
Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna son, Okinawa 904-0495, Japan
Shu-hua Lee
Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10, Dah-Uen Rd., Jiau Shi, I-Lan 262, Taiwan
Simon de Bernard
Altrabio SAS, 30 Rue Pré-Gaudry, 69007 Lyon, France
Mathieu Reynaud
Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna son, Okinawa 904-0495, Japan
Pauline Salis
Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, Observatoire Océanologique, 66650 Banyuls-sur-Mer, France
Agneesh Barua
Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna son, Okinawa 904-0495, Japan
Abdelhay Boulahtouf
Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM, University of Montpellier, 34090 Montpellier, France
Patrick Balaguer
Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM, University of Montpellier, 34090 Montpellier, France
Karine Gauthier
Institut de Génomique Fonctionnelle de Lyon, CNRS UMR 5242, INRAE USC 1370 École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, 46 allée d’Italie, 69007 Lyon, France
David Lecchini
PSL Research University, EPHE-UPVD-CNRS-UAR 3278 CRIOBE BP 1013, 98729 Papetoai, Moorea, French Polynesia; Laboratoire d’Excellence “CORAIL,” 66100 Perpignan, France
Yann Gibert
Department of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA
Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna son, Okinawa 904-0495, Japan; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10, Dah-Uen Rd., Jiau Shi, I-Lan 262, Taiwan; Corresponding author
Summary: Most marine organisms have a biphasic life cycle during which pelagic larvae transform into radically different juveniles. In vertebrates, the role of thyroid hormones (THs) in triggering this transition is well known, but how the morphological and physiological changes are integrated in a coherent way with the ecological transition remains poorly explored. To gain insight into this question, we performed an integrated analysis of metamorphosis of a marine teleost, the false clownfish (Amphiprion ocellaris). We show how THs coordinate a change in color vision as well as a major metabolic shift in energy production, highlighting how it orchestrates this transformation. By manipulating the activity of liver X regulator (LXR), a major regulator of metabolism, we also identify a tight link between metabolic changes and metamorphosis progression. Strikingly, we observed that these regulations are at play in the wild, explaining how hormones coordinate energy needs with available resources during the life cycle.
