PLoS ONE (Jan 2011)

Rapid screening of complex DNA samples by single-molecule amplification and sequencing.

  • Jiaqi Huang,
  • Zongli Zheng,
  • Anders F Andersson,
  • Lars Engstrand,
  • Weimin Ye

DOI
https://doi.org/10.1371/journal.pone.0019723
Journal volume & issue
Vol. 6, no. 5
p. e19723

Abstract

Read online

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.