Frontiers in Plant Science (Oct 2017)
OsGIF1 Positively Regulates the Sizes of Stems, Leaves, and Grains in Rice
- Zhongshan He,
- Zhongshan He,
- Jing Zeng,
- Jing Zeng,
- Yun Ren,
- Yun Ren,
- Dan Chen,
- Dan Chen,
- Wenjie Li,
- Wenjie Li,
- Fengyan Gao,
- Fengyan Gao,
- Ye Cao,
- Ye Cao,
- Tao Luo,
- Tao Luo,
- Guoqiang Yuan,
- Guoqiang Yuan,
- Xianghong Wu,
- Xianghong Wu,
- Yueyang Liang,
- Yueyang Liang,
- Qiming Deng,
- Qiming Deng,
- Shiquan Wang,
- Shiquan Wang,
- Aiping Zheng,
- Aiping Zheng,
- Jun Zhu,
- Jun Zhu,
- Huainian Liu,
- Huainian Liu,
- Lingxia Wang,
- Lingxia Wang,
- Ping Li,
- Ping Li,
- Ping Li,
- Shuangcheng Li,
- Shuangcheng Li
Affiliations
- Zhongshan He
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Zhongshan He
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Jing Zeng
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Jing Zeng
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Yun Ren
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Yun Ren
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Dan Chen
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Dan Chen
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Wenjie Li
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Wenjie Li
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Fengyan Gao
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Fengyan Gao
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Ye Cao
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Ye Cao
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Tao Luo
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Tao Luo
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Guoqiang Yuan
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Guoqiang Yuan
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Xianghong Wu
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Xianghong Wu
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Yueyang Liang
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Yueyang Liang
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Qiming Deng
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Qiming Deng
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Shiquan Wang
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Shiquan Wang
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Aiping Zheng
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Aiping Zheng
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Jun Zhu
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Jun Zhu
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Huainian Liu
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Huainian Liu
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Lingxia Wang
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Lingxia Wang
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Ping Li
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Ping Li
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- Ping Li
- State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, China
- Shuangcheng Li
- State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, China
- Shuangcheng Li
- Rice Research Institute, Sichuan Agricultural University, Wenjiang, China
- DOI
- https://doi.org/10.3389/fpls.2017.01730
- Journal volume & issue
-
Vol. 8
Abstract
Growth-regulating factor (GRF) interacting factors (GIFs) are involved in several developmental processes in Arabidopsis. We previously showed that upregulation of OsGIF1 expression improves rice grain size. However, whether OsGIF1 is involved in other developmental processes remains unclear. Here, we report pleiotropic effects of OsGIF1 on rice organ size regulation. Overexpression and functional knock-out via a CRISPR/Cas9 strategy revealed that OsGIF1 not only positively regulates the sizes of rice leaf, stem, and grain but also influences rice reproduction. Expression profiles based on both qRT-PCR and GUS (β-glucuronidase) histochemical staining suggested that OsGIF1 is differentially expressed across various rice tissues, consistent with its roles in regulating the development of multiple rice organs. Additionally, we found that OsGIF1-GFP localized preferentially in the nucleus, which supports its proposed role as a transcriptional cofactor. Further histological analysis suggested that OsGIF1 affected rice organ size possibly by regulating cell size. Our results suggest that OsGIF1 plays important roles in vegetative and reproductive developmental processes, with important implications for rice breeding.
Keywords
