Validation of the method for determining the specific activity of the gel with recombinant endolysin

Abstract

A method for determining the specific bactericidal activity of the gel with the recombinant modified endolysin of the bacteriophage ECD7 has been developed. Bacterial strains to determine the specific bactericidal activity were selected and acceptance criteria were determined. The developed methodology was validated according to the indicators “Specificity”, “Accuracy” and “Intermediate precision”. The developed method has been successfully validated and is suitable for determining the specific bactericidal activity of a gel with a recombinant modified endolysin.

Keywords

• bactericidal activity
• recombinant endolysin
• bacteriophage ecd7